Origins, actions and dynamic expression patterns of the neuropeptide VGF in rat peripheral and central sensory neurones following peripheral nerve injury

doi:10.1186/1744-8069-4-62

Molecular Pain

Volume 4

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Moss A
Ingram R
Koch S
Theodorou A
Low L
Baccei M
Hathway GJ
Costigan M
Salton SR
Fitzgerald M

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Ingram R
Koch S
Theodorou A
Low L
Baccei M
Hathway GJ
Costigan M
Salton SR
Fitzgerald M
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Origins, actions and dynamic expression patterns of the neuropeptide VGF in rat peripheral and central sensory neurones following peripheral nerve injury

Andrew Moss¹^,4 , Rachel Ingram¹ , Stephanie Koch¹ , Andria Theodorou¹ , Lucie Low¹ , Mark Baccei¹ , Gareth J Hathway¹ , Michael Costigan² , Stephen R Salton³ and Maria Fitzgerald¹

¹UCL Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK

²Neural Plasticity Research Group, Department of Anesthesia & Critical Care, Mass General Hospital & Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA

³Fishberg Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA

⁴Pfizer Global Research & Development, Pain Therapeutics, Ramsgate Road, Sandwich, Kent, CT13 9NJ, UK

author email corresponding author email

Molecular Pain 2008, 4:62doi:10.1186/1744-8069-4-62


Published:	10 December 2008

Additional files

Additional file 1:

Origins of terminal staining of VGF in the lumbar spinal cord. A. Immunohistochemistry of VGF (left) and CGRP (right) in the L4 spinal cord following unilateral L3–5 dorsal rhizotomy 5 days earlier. Ipsilateral CGRP depletion indicates total loss of primary afferent input. VGF staining in the region is decreased but not totally depleted (arrow). B. VGF (left) and 5 HT (right) immunostaining in L4 spinal cord following unilateral lesion of the dorsolateral funiculus at upper thoracic level 5 days earlier. Ipsilateral 5-HT depletion indicates loss of descending brainstem terminals, while VGF is only partially diminished (arrow). C Retrograde labeling of projection neurones in the rostroventral medulla, using bilateral True blue (2%) injection into the L4/5 spinal cord under anaesthetic, 5 days earlier (red), demonstrates expression of VGF in brainstem descending projection neurones. True blue:red. VGF:green. Double labeled: orange.

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Additional file 2:

Origins of intrinisic staining of VGF in the lumbar spinal cord. A Immunohistochemistry for VGF and NeuN highlighting the ipsilateral increase in VGF in intrinsic dorsal horn neurones (Arrow). B High-power confocal immunohistochemistry of VGF and markers for microglia (Iba-1), neurones (NeuN) and astrocytes (GFAP) demonstrates that intrinsic VGF protein expression is co-localised specifically with the neuronal marker NeuN but not with either of the glial markers (arrows).

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