Figure 3.

Immunohistochemistry of VGF in the spinal cord of (A) naïve and (B) nerve injured rats at 7 days and (C) 21 days post-SNI. Arrows show increased VGF staining in the C fibre terminal region of the ipsilateral superficial laminae (LI-II), intrinsic dorsal horn neurons throughout the dorsal horn and ventral horn neurons (n = 4 per group). D. Increased VGF expression in the central afferent terminals (arrow) coincides with afferent IB4 depletion following SNI. (Scale bars: 100 μm). E. Double immunolabeling of naïve (uninjured) spinal cord sections shows colocalisation in terminals (yellow, arrow) of VGF (green) and CGRP (red) a marker of peptidergic C-fibres. F. Double immunolabeling of naïve uninjured spinal cord sections shows little overlap in VGF (green, arrow) and IB4 (red), a marker of non-peptidergic C-fibres (Scale bars: 50 μm).