Additional file 2.Origins of intrinisic staining of VGF in the lumbar spinal cord. A Immunohistochemistry for VGF and NeuN highlighting the ipsilateral increase in VGF in intrinsic dorsal horn neurones (Arrow). B High-power confocal immunohistochemistry of VGF and markers for microglia (Iba-1), neurones (NeuN) and astrocytes (GFAP) demonstrates that intrinsic VGF protein expression is co-localised specifically with the neuronal marker NeuN but not with either of the glial markers (arrows). Format: PPT Size: 2.8MB Download file This file can be viewed with: Microsoft PowerPoint Viewer Moss et al. Molecular Pain 2008 4:62 doi:10.1186/1744-8069-4-62 |